SYBR Green I - Définition

Bibliographie

Bibliographie francophone

Bibliographie anglophone

• Ames BN, McCann J, Yamasaki E, Methods for detecting carcinogens and mutagens with the Salmonella/mammalian-microsome mutagenicity test. 1975. Mutat. Res. 31:347–364.
• Bachoon DS, Otero E, Hodson RE. Effects of humic substances on fluorometric DNA quantification and DNA hybridization. 2001. J. Microbiol. Methods, 47:73–82.
• Brussaard CP, Marie D, Bratbak G. Flow cytometric detection of viruses. 2000. J. Virol. Methods, 85:175–182
• Jin X, Yue S, Wells KS, Singer VL, SYBRTM Green I: a new fluorescent dye optimized for detection of picogram amounts of DNA in gels. 1994. Biophys. J. 66:A159.
• Jin X, Yue S, Singer VL, Highly sensitive gel assays for detecting nucleases. 1995. FASEB J. 9:A1400.
• Kiltie AE, Ryan AJ. SYBR Green I staining of pulsed field agarose gels is a sensitive and inexpensive way of quantitating DNA double-strand breaks in mammalian cells. 1997. Nucleic Acids Res. 25:2945–2946.
• Maron DM, Ames B. Revised methods for the Salmonella mutagenicity test. 1983; Mutat. Res. 113:173–215.
• Morin PA, Smith DG, Nonradioactive detection of hypervariable simple sequence repeats in short polyacrylamide gels. 1995. BioTechniques 19:223–227.
• Ohta T, Tokishita S, Yamagata H. Ethidium bromide and SYBR Green I enhance the genotoxicity of UV-irradiation and chemical mutagens in E. coli. 2001. Mutat Res. May 31;492(1-2):91-7.
• Rye HS, Yue S, Wemmer DE, Quesada MA, Haugland RP, Mathies RA, Glazer AN. Stable fluorescent complexes of double-stranded DNA with bis-intercalating asymmetric cyanine dyes: properties and applications. 1992. Nucleic Acids Res. 20:2803–2812.
• Schneeberger CS, Speiser P, Kury F, Zeillinger R. Quantitative detection of reverse transcriptase–PCR products by means of a novel and sensitive DNA stain, PCR Methods. 1995. Appl. 4 :234–238.
• Singer VL, Jin X, Ryan D, Yue S. SYBRTM Green dyes: ultrasensitive stains for detection of DNA and RNA in electrophoretic gels. 1994. Biomed. Products 19:68–72.
• Singer VL, Lawlor TE, Yue S. Comparison of SYBR Green I nucleic acid gel stain mutagenicity and ethidium bromide mutagenicity in the Salmonella/mammalian microsome reverse mutation assay (Ames test). 1999. Mutat Res 439:37–47.
• Skeidsvoll J, Ueland PM, Analysis of double-stranded DNA by capillary electrophoresis with laser-induced fluorescence detection using the monomeric dye SYBR Green I. 1995. Anal. Biochem. 231:359–365.
• Yasuda T, Takeshita H, Nakazato E, Nakajima O, Hosomi O, Nakashima Y, Kishi K, Activity measurement for deoxyribonucleases I and II with picogram sensitivity based on DNA SYBR Green I fluorescence. 1998. Anal. Biochem. 255:274–276.
• Zipper H, Brunner H, Bernhagen J, Vitzthum F. Investigations on DNA intercalation and surface binding by SYBR Green I, its structure détermination and methodological implications. 2004. Nucl Acids Res; 32:e103.

Toxicité et consigne de sécurité

Le SYBR Green présente une mutagenicité non nulle mais cependant très inférieure au Bromure d'éthidium. Les précautions d'usage doivent être néanmoins conservées.